Surface Particles of Sarcoplasmic Reticulum Membranes
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چکیده
منابع مشابه
Surface Particles of Sarcoplasmic Reticulum Membranes
Particles 30 to 40 A in diameter are attached to the external surface of negatively stained membrane vesicles formed by the ATPase of sarcoplasmic reticulum. These particles are identical with the surface particles of isolated, intact sarcoplasmic reticulum first described by Ikemoto et ~2. (IKEMOTO, N., SRETER, F. A., AND GERGELY, J. (1966) Fed. Proc. 25, 465). Tryptic digestion was used in an...
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Fragmented sarcoplasmic reticulum (FSR) vesicles from rabbit muscle were suspended in 1.5-5% glycerol solutions and were pelleted onto aluminum foil disks in a modified centrifuge tube. Examination of these pellets in the electron microscope after drying for 2--2.5, 4--5.5, and 21 hours revealed a progression of changes. First, distances between individual, round vesicles decreases. Next, somew...
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During the excitation of muscle the estimated rate of Ca2+ release from sarcoplasmic reticulum may increase 10(3)- to 10(4)-fold compared with relaxed muscle or isolated sarcoplasmic reticulum in vitro, implying a major change in the calcium permeability of the sarcoplasmic reticulum membrane. As a first step in the assessment of the role of various membrane constituents in the regulation of ca...
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It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol ...
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Fragmented sarcoplasmic reticulum (FSR) membranes isolated from rabbit skeletal muscle are impermeable to inulin-(14)C (mol wt 5,000), and dextran-(14)C (mol wt 15,000-90,000) at pH 7.0-9.0, yielding an excluded space of 4-5 microl/mg microsomal protein. In the same pH range urea and sucrose readily penetrate the FSR membrane. EDTA or EGTA (1 mM) increased the permeability of microsomes to inul...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43028-7